The structure of an essential splicing element: stem loop IIa from yeast U2 snRNA
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چکیده
منابع مشابه
The structure of an essential splicing element: stem loop IIa from yeast U2 snRNA.
BACKGROUND Eukaryotic genes are usually transcribed as precursor mRNAs which are then spliced, removing introns to produce functional mRNAs. Splicing is performed by the spliceosome and provides an important level of post-translational control of gene expression. Stem loop IIa from U2 small nuclear (sn)RNA is required for the efficient association of the U2 small nuclear ribonuclear protein (sn...
متن کاملInvariant U2 snRNA nucleotides form a stem loop to recognize the intron early in splicing.
U2 snRNA-intron branchpoint pairing is a critical step in pre-mRNA recognition by the splicing apparatus, but the mechanism by which these two RNAs engage each other is unknown. Here, we identify a U2 snRNA structure, the branchpoint-interacting stem loop (BSL), which presents the U2 nucleotides that will contact the intron. We provide evidence that the BSL forms prior to interaction with the i...
متن کاملThe intramolecular stem-loop structure of U6 snRNA can functionally replace the U6atac snRNA stem-loop.
The U6 spliceosomal snRNA forms an intramolecular stem-loop structure during spliceosome assembly that is required for splicing and is proposed to be at or near the catalytic center of the spliceosome. U6atac snRNA, the analog of U6 snRNA used in the U12-dependent splicing of the minor class of spliceosomal introns, contains a similar stem-loop whose structure but not sequence is conserved betw...
متن کاملMutation in the U2 snRNA influences exon interactions of U5 snRNA loop 1 during pre-mRNA splicing.
The U2 and U6 snRNAs contribute to the catalysis of intron removal while U5 snRNA loop 1 holds the exons for ligation during pre-mRNA splicing. It is unclear how different exons are positioned precisely with U5 loop 1. Here, we investigate the role of U2 and U6 in positioning the exons with U5 loop 1. Reconstitution in vitro of spliceosomes with mutations in U2 allows U5-pre-mRNA interactions b...
متن کاملStructure and thermodynamics of a conserved U2 snRNA domain from yeast and human.
The spliceosome is a dynamic ribonucleoprotein complex responsible for the removal of intron sequences from pre-messenger RNA. The highly conserved 5' end of the U2 small nuclear RNA (snRNA) makes key base-pairing interactions with the intron branch point sequence and U6 snRNA. U2 stem I, a stem-loop located in the 5' region of U2, has been implicated in spliceosome assembly and may modulate th...
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ژورنال
عنوان ژورنال: Structure
سال: 1997
ISSN: 0969-2126
DOI: 10.1016/s0969-2126(97)00268-2